The (pre-semester) microscopy course is organized by the Imaging & Optics Facility (IOF) and aims to generate the understanding of how to design your experiments using matching imaging techniques. As a red line we raise awareness of the importance of proper data acquisition and the timely image analysis assessments during essay establishment: timely assay-evaluation is critical to determine if the fundamental ‘properties of process of interest’ can be resolved and quantified by the used imaging approach. To this aim, participants will be introduced to contemporary microscopy techniques, with a focus on the methods that are available at ISTA campus. Lectures will cover microscopy-related principles of optics, principles of fluorescence, anatomy of the microscope, sample preparation, methods of optical sectioning, image acquisition methods, super-resolution technologies, basics of electron microscopy and the basics of data analysis.
SCHEDULE:
The Imaging & Optics Facility is providing the ‘Annual Microscopy Course’ – split over 2 weeks:
– week 1:
Sep 23 (Monday) 9.00-13.00 – Mondi 3
Sep 24 (Tuesday) 9.00-17.15 – Mondi 3
Sep 24 (Friday) 13.00-17.15 – Mondi 3
– week 2:
Sept 25 (Monday) 9.00-12.30 – Mondi 3
Oct 1 (Tuesday) 9.00-12.30 – Mondi 3
Oct 2 (Wednesday) 9.00-12.30 – Mondi 3
Oct 3 (Thursday) 9.00-12.30 – Mondi 3
Oct 4 (Friday) 9.00-12.30 – Mondi 3
PRELIMINARY detailed PROGRAM:
wk
date
start
end
type
groups 1
groups 2
groups 3
groups 4
week 1
Mon Sept 23
9:00
9:30
welcome
Intro to facility & course organization
9:30
10:45
lecture / demo
Anatomy of the microscope
10:45
12:00
lecture / demo
Intro to Fluorescence
Tues Sept 24
9:00
10:30
lecture (1h30)
Optical sectioning
10:30
11:59
lecture (1h30)
Scientific cameras
12:01
12:45
lunch (0h45)
13:00
14:55
System-demo (1h55)
widefield / TIRF
widefield / TIRF
Cytometry
Cytometry
14:55
15:05
change-break
change at Mondi 3
15:05
17:00
System-demo (1h55)
Cytometry
Cytometry
widefield / TIRF
widefield / TIRF
Fri, Sept 27
confocal
13:00
14:30
lecture (1h30)
Intro to optics
14:30
14:45
change-break
change at Mondi 2
14:45
17:00
lecture (1h30)
Sample preparation
week2
Mon, Sept 30
confocal
9:00
10:30
System-demo (1.5h)
Confocal & Airyscan
Confocal & Airyscan
Spinning Disk
Spinning Disk
10:30
10:45
change-break
change at Mondi 2
10:45
12:30
System-demo (1.5h)
Spinning Disk
Spinning Disk
Confocal & Airyscan
Confocal & Airyscan
Tue Oct 1
Advanced tech.
9:00
10:30
System-demo (1.5h)
light sheet
MP
10:30
10:45
change-break
change at Mondi 2
10:45
12:30
System-demo (1.5h)
MP
light sheet
Wed Oct 2
High res.
9:00
10:30
lecture (1h30)
Super-resolution technologies & Deconvolution
10:30
12:30
lecture (1h30)
Intro to Electron Microscopy
Thu, Oct 3
13:00
14:00
lecture
What is a digital image?
14:10
15:25
demonstration
Intro to image analysis (basics of FIJI)
15:30
16:30
lecture
ImageJ / FIJI: image analysis
Fri, Oct 4
13:00
14:30
lecture
Machine learning in image analysis
14:45
16:20
demonstration
What to use for what: Arrivis / Imaris / Huygens / Zen / JOBS …
16:20
16:30
recap
recap & exam details
REGISTRATION
Details for registration for the course will be made available on the GraduateSchool ISTA Athena.
‘Students’ join for 2 ECTS credits and will have manditory participation in all lectures, on-system demonstrations (100% attendance required) and require to complete an exam at the end of the course (max 12 students)
‘Audit Students’ can join lectures and we will fill up vacant hands-on spots by order of registration (Zoom links will be shared to registered participants)
All lectures can be followed in hybrid: credit-students attendance required!
