Zeiss LSM-07 (900up_Airyscan 2)

Location: I04.U1.015

Axio Imager.Z2 Upright LSM900 confocal microscope + Airyscan 2, suited for live imaging: equipped with heating chamber, ZEN 3.8 with experiment designer, sample navigator and macro-environment (OAD).

The system is also equipped with an Airyscan 2 module incl. multiplex-mode: readout of up to 4 pixels at once while retaining super resolution (~140nm in X and Y), resulting in highly increased speed and more gentle imaging, especially important for live samples.

This confocal microscope is well suited for life imaging: book in PPMS– 4h limit in core hours

Sample conditioning:

  • Heating chamber

Light sources:

  • Laser lines 405, 488, 561, 640 nm
  • Colibri 7 LED unit for visual examination of dyes in the blue, green, orange/red and far red spectra
  • VIS – LED for transmitted light

Detectors: 3 +1 channels simultaneously (more sequentially)

  • 2x GaAsP PMTs: Gallium Arsenide Phosphide PhotoMultiplier Tube detectors with free choice of the spectral range by using 2 variable beam splitter dichroics and additional filters
  • 1 x Airyscan2 detector – optimized for 40x objective. Can perform multiplexing, or can be used as 3rd GaAsP detector!
  • 1x T-PMT + LED light source: Electronically switchable illumination and detection module

Motorized stage for multiple position imaging & Sample Navigator

  • Dish holder, single slide holder. NO multi-slide holder available!

Objectives (M27 thread)

  1. EC Plan-Neofluar 2,5x/0,085 M27(420320-9902-000)
  2. W Plan-Apochromat 20x/1,0 DIC D=0,17 M27 75mm (421452-9880-000)
  3. Objective Plan-Apochromat 40x/1,3 Oil DIC M27
    (420762-9800-799) – € – special selection for Airyscan
  4. Objective Plan-Apochromat 63x/1,4 Oil DIC M27 (420782-9900-799) – € – special selection for Airyscan

Not mounted objectives:

  • Plan-Apochromat 20x/0,8 M27 Air (420650-9902-000)
  • ACR – Objective Plan-Apochromat 63x / NA 1.4 Oil
  • ACR – Plan APOCHROMAT 40x/1.1 Water
  • ACR – Plan APOCHROMAT 5x/0.16 Air

Software: Zen 3.8

  1. ZEN Module Sample Navigator
  2. ZEN Module Advanced Processing
  3. ZEN Module Macro Editor
  4. ZEN Module Experiment Designer Hardware
  5. ZEN Module Tiles & Positions
  6. ZEN Direct processing

Computer: HP Z6

  • Intel® Xeon™ Gold 6134 CPU @ 3.0GHz + 2GHz (2 processors)
  • NVIDIA Quadro P4000, 8Gb
  • 192 GB RAM
  • 8TB RD10 data drive
  • 10 Gbit network connection

Contact : Imaging and Optics Facility

System manual and additional material is available on SeaFile (login required).