Coming soon!
Location: I06.U1.405
The Lattice Light Sheet Microscope (LLSM) is designed for long-term volumetric imaging at sub-cellular resolution. The system allows standard sample carriers, and is equipped with temperature, humidity and CO2 control. The Lattice Lightsheet technologies reduces phototoxicity and photobleatching, allowing monitoring of cellular processes for extended period (hours – to – days).
Specification
- Multi-position & Tiling
- Versatile chambers and sample holders, with temperature, humidity and CO2 control.
- Additional piezo driven z-axis for fast imaging
- Transmitted Light (+ enhanced oblique contrast)
- Dual camera (2 x Hamamatus Orca Fusion sCMOS)
- Lattice Light sheets (typical): 500nm x 12µm ;650nm x 15µm; 750nm x 30µm; 1000nm x 30µm;1400nm x 85µm; 2000nm x 100µm
- Lateral resolution: 330nm x 330nm (+ Deconvolution: 290nm x 290n)
- Axial resolution(dependingon lightsheet): ranging from500nm to1000nm (+ Deconvolution: ranging
from450nm and 900nm)
Objectives
The system is equipped with dedicated optics, and an automated water-immersion dispenser
- Excitation objective: 10x / 0.4, Water Immersion
- Meniscus lens
- detection objective: 48x / 1.0, Water Immersion + motorized coverslip adjustment
Schematic below show the sample carrier and core optics module with excitation objective (1), meniscus lens (2) and detection objective with free-form optics (3).
Sample mounting
The system is optimized for conventional sample preparation techniques, and is quipped with temperature; CO2, O2, humidity -control. Please take not to use the correct cover slips (#1.5) !
- holder #1: pertridish 35-40mm
- holder #2: chamber slides 25 x 57mm
- holder #2: multiwell plates 85 x 127 mm
Laser lines
The Lattice Light Sheet 7 is configured with the “high-laserpower laser module” to allow more flexibility and explore SMLM applications
- 488nm (14mW)
- 561nm (9mW)
- 638nm (13mW)
Filters / Beam-splitters
Dichroic / camera splitter:
- LP 565
- LP 640
Camera 1:
-
BP 570-620 + LP655
-
BP 495-550 / BP 570-620
-
BP 495-550 + LP655
-
LBF 405/488/561/642
-
BP 495-590
-
LP 570
-
1%
Camera 2:
-
- BP 570-610 IR+
- BP 495-550 / BP 570-620
- BP 500-550 IR+
Camera’s
- 2x Hamamatsu ORCA-Fusion sCMOS (> 82% QE und 6.5 um Pixelsize)
Resources: