Automated Widefield Fluorescent Microscope
Location: i23.U1.015 – book in ppms
Specifications:
- Motorized stage, with multi-position option
- Time lapse option,
- Color and monochrome cameras
- phase contrast imaging with 10x, 20x, 60x oil, 100x oil objectives
Objectives:
- 4x/0.2 air
- 10x/0.3 PH1 air
- 20x/0.5 PH1 air
- CFI Plan Apo Lambda 40XC/0.95Air – WD: 0.25–0.16 mm
- 60x/1.4 PH3 oil
- 100x/1.45 PH3 oil
Emission filters:
-
- DAPI (LP 409, BP 447/60)
- Piston GFP (LP 495, BP 520/35)
- TexasRed HYQ=mCherry (LP 596, BP 641/75)
- YFP HYQ (LP520, BP 542/27),
- CFP (LP458, BP 483/32)
- optional: Far Red (LP660, BP692/40)
Lumencor light source with flexible excitation band selection:
-
- Violet (390/18)
- Blue (438/24)
- Cyan (475/28)
- Teal (513/17)
- Green (549/15) (always in combination with Yellow)
- Yellow (575/25) (always in combination with Green)
- Red (632/22)
Contact: Imaging & Optics Facility
Manuals: