The Imaging and Optics Facility is facilitating the use and maintenance of an wide variety of advanced Microscopy, Imaging analysis and Cytometry devices, as well as software licenses and system accessories. Before anyone can make use of any system, users need to complete a training by the Imaging and Optics Facility Facility team before bookings can be made.
Grouped Equipment Overview:
The Imaging and Optics Facility provides an high-end Flow Cytometry machine park and analysis infrastructure.
Our widefield equipment park consists of automated widefield microscopes, as well as slidescanners. The slidescanner are best used for large volume imaging; the inverted Nikon Ti2E microscopes allow diverse options for automation routines – using JOBS, and have been equipped with incubators chambers, CO2 gas mixers and a variety of (custom) stage inserts. the Zeiss upright systems – and the Axioscan Slide scanner service – excell for quick scans and making overiew images that can be used on confocal – using ZEN Connect. Luminescent samples can be images on our dark-box.
Confocal laser scanning microscopy is a flexible technology that suite many imaging applications. Our confocal machine park consists a diverse set of confocals micrscopes in upright (↓), inverted (↑) and vertical (↔) orientation. Different applications are available, such as Airyscan, FRAP, FLIM, as well as options to automation routines, such as ‘experiment designer’. Using ZEN Connect, widefield image data (e.g. slidescanner images) can be opened and navigated trough to assist setup of imaging, or add multidimnetional data-sets. Many of the confocal setups have been equipped with incubators or (custom) heated-controlled stage inserts and/or CO2 gas mixers. A dual pipette Aspiration assay has been added to the Leica sp5 inverder microscope.
Multiphoton microscopy relies on the simultaneous absorption of two or more photons by a fluorophore, most often using infra-red laser. Multiphoton microscopy most significan atvanteage over other technoligies is that it allows exceptionally deep light penetration with less phototoxicity. It therefore best used for ‘non-invasive’ imaging in living samples / organism. Our multiphoton system allows a very large working space, resonance scanner and allows Fluorescent Life-Time imaging applications (FLIM).
Spinning Disk microscopes – also known as ‘Multi-point Confocal microscope’ are fast camera-based imaging systems. Spining Disk Confocals technology induces low phototoxicity and reduced photo-bleaching effects compared to point scanning micrscopes and are therefore a good match for live imaging but als excell in aquisition of large data volumes (cleared tissues, expanded samples or brain sections). Most systems have been equipped with incubators chambers or temperature-contolled stages inserts, CO2 gas mixers and/or a variety of (custom) stage inserts. In addition, Spinning-disk confocal machine park allows many options to cusomize automation routines, as well as feed-back routines allow the triggering of external hard-ware during aquisition → please approach for custom automation project request.
‘Multi-modal multi-point confocal system‘ are also fast camera-based imaging system – similar to our Spining Disk Confocal Micrscopes – but host additional modalities, such as Widefield, Super-resolution (SRRF, SoRa) or TIRF modalities. Different modalities can be combined in both live and fixed samples of different volumes. The systems have been equipped with temperature incubators, CO2 gas mixers and/or a variety of (custom) stage inserts.
Total Internal Reflection Fluorescence (TIRF) Microscopy uses the properties of an induced evanescent wave – by exciting the sample under a ‘critical angle’ – immediately adjacent to the interface between two media having different refractive indices (RI): the contact area between the specimen (e.g. liquid, cells) and the glass coverslip or culture dish. This means that fluorophores are only excted in a very limited volume, also increase in signal-to-noise ratio from the restricted of the excitation volume. TIRF is mostly applied for ‘high resolution’ imaging application and is often comined with fluorescence photobleaching recovery (FRAP). All our TIRF setups have enclosed interlocked incubation chambers to guarentee laser safety. Most systems are equipped with temperature incubator and CO2 gas mixers.
Lightsheet microscopy – otherwise known as selective plane illumination microscopy (SPIM) is a powerful technique for (large) volumetric imaging data with isotropic resolution. Light sheet microscopy is a fast cameras based imaging application that restricts photobleaching and phototoxicity effects. Due to these benefits, Light Sheet micrscopy is often used for in-depth analyses of large, optically cleared (or expanded) samples and for long-term three-dimensional (3D) observations of live biological specimens at high spatio-temporal resolution.
OpenSPIM Light Sheet Microscope (room i06.U1.405): RETIRED
- Zeiss Light Sheet 7 Microscope (room i06.U1.405): implementation 2022
As part of the Imaging and Optics Facility infrastructure, a number of stereo microscope are available for sample-inspection and -mounting near to our imaging setups. Two stereo microscopes are equipped with an monochrome camera.
The Imaging and Optics Facility provides an sate-of the arte image analysis infrastructure that includes a large number of high-end analysis workstations as well as a broad spectrum of different image analysis software applications.
In addition, we provide project based solutions for custom-tailored image analysis tasks: we help you to automate your image analysis workflow, or establish new image analysis routines using the image analysis programs available.
Workstations machine park consits of 9 PCs and 5 Virtual Machines (VDI), where VDI machines are based on an ‘golden image’ – that is shared over multiple workstations. Analysis workstations are specified for specific image analysis applications: ‘classical image analysis‘ & ‘machine learning-based‘ analysis. All our workstation can be accessed from remote, and a large portion can also be used from dedicated ‘connection terminals’ in room i01.O3.52a/b.
Workstations (both PCs and VDIs) have preinstalled software, where some ‘propriety software’ will need to be booked seperately – as resources are limited by the number of lisences available.
In addition, we provide project based solutions for custom-tailored image analysis tasks: we help you to automate your image analysis workflow, or establish new image analysis routines using the image analysis programs available*.
AUTOMATED RENTAL SYSTEM
The Imaging and Optics facility released an “Automated Rental System” for Experimental Accessories, Optical Tools, (high-end) Objectives. The Automated Rental System helps to coordinate access-rights and the utilization of the available rental items and allows the Imaging and Optics facility to make the rentals available during the weekends → A brief trainings is required to get booking rights.
Retired systems are not available anymore, but the pages are kept for system information