Nikon Eclipse Ti Inverted

Automated Widefield Fluorescent Microscope

Location: i23.U1.015 – book in ppms

Specifications:

  • Motorized stage, with multi-position option
  • Timelapse option
  • Colour and monochrome cameras
  • Phase contrast imaging with 10x, 20x, 60x oil, 100x oil objectives

Objectives:

  1. 4x/0.2 air
  2. 10x/0.3 PH1 air
  3. 20x/0.5 PH1 air
  4. CFI Plan Apo Lambda 40XC/0.95Air – WD: 0.25–0.16 mm
  5. 60x/1.4 PH3 oil
  6. 100x/1.45 PH3 oil

Emission filters:

    • DAPI (LP 409, BP 447/60)
    • Piston GFP (LP 495, BP 520/35)
    • TexasRed HYQ=mCherry (LP 596, BP 641/75)
    • YFP HYQ (LP520, BP 542/27),
    • CFP (LP458, BP 483/32)

CoolLED pE300 White fluorescence lightsource 

    • Violet (390/18)
    • Blue (438/24)
    • Green (549/15) (always in combination with Yellow)

System manual and additional material is available on SeaFile (login required).

Contact: Imaging & Optics Facility