Location: room i04.U1.015 – book in PPMS
The Nikon CSU W1-01 SoRa+NIR spinning disk microscope is an advanced enhanced resolution imaging modality, and the future (!) platform of the 355 nm UV laser ablation system. It has a piezo drive for fast and precise imaging of 3D stacks, and is equipped with an incubation chamber and a CO2/O2 gas mixer.
Imaging modalities/features
- Confocal (spinning disk)
- Widefield (CoolLED)
- Super resolution via optical Re-assignment (SoRa)
- NIR imaging (not available in SoRa mode)
- Feedback microscopy
Specifications
- Nikon Ti2E inverted microscope (double deck) with Perfect Focus System (PSF) / widefield
- Yokogawa CSU-W1 dual-disk spinning disk unit with 50 μm pinholes & 50 μm pinholes with micro-lenses SoRa disk
- Yokogawa SoRa with magnification changer for optimum resolution across different objective magnifications (for SoRa modality)
- 4.0x; 60x objective; 55×55 μｍ FOV (40x objectives also possible with limited resolution gain)
- 2.8x; 100x objective; 48×48 μｍ FOV
- 1.0x; normal spinning disk imaging
- Resolution
| SoRa mode (optical only) | SoRa mode + deconvolution | |
| XY resolution | 150 nm | 120 nm |
| Z resolution | 320 nm | 300 nm |
- 2 Teledyne Photometrics BSI cameras
- Motorized stage:
- Nikon TI2-S-SE-E XY stage with linear encoders: 100 nm XY resolution, 500 nm XY accuracy
- Mad City Labs Z piezo & linear stage encoder: travel range: 200 μm, 0.4 nm min step size
- Incubation chamber: IST custom-built dark chamber with Life Imaging Services heating unit (25-42 ºC)
- Ibidi CO2/O2 gas mixer
Light sources
- CoolLED – pE-300 White Light LED source
- Visible lasers: Omicron LightHUB UltraN
- 405 nm laser: (120 mW) – Continuous wave
- 488 nm laser: (200 mW) – Continuous wave
- 561 nm laser: (150 mW) – Continuous wave
- 638 nm laser: (200 mW) – Continuous wave
- NIR laser: Omicron LightHUB NIR
- 785 nm laser: (200 mW) – Continuous wave
Filters for visual/widefield examination (lower deck)
- DAPI-5060C Filter Cube: Ex 377/50, DM 409, Emission BP 447/60
- GFP-4050B Filter Cube: Ex 466/40, DM 495, Emission BP 525/50
- mCherry-C Filter Cube: Ex 562/40, DM 593, Emission BP 640/75
- Cy5-4040C Filter Cube: Ex 628/40, DM 660, Emission BP 692/40
Objectives / position
- CFI Plan Apo λ 10x air / NA 0.45 / WD 4 mm (MRD00105)
- CFI Plan Apo λ 40x air / 0.95/ 0.17-0.25 mm (MRD00405)
- CFI Plan Apo VC 60x WI/ NA 1.2 / WD 0.28-0.31 mm / Water – (MRD07602) – €€
- CFI Plan Apo λ 60x oil / NA 1.4/ WD 0.13 mm / oil (MRD01605) – €€
- CFI SR HP Plan Apo λ 100x Sil / NA 1.35/ WD 0.30 mm / Silicone oil (MRD73950) – €€€
- Free slot for bookable objectives
Light path and cameras
Dichroic Mirror Block
- Confocal (visible lasers) quad-band dichroic mirror: 405 nm / 488 nm / 561 nm / 640 nm
- Widefield (CoolLED) dichroic mirror: 350-700 nm
- Near InfraRed (NIR laser) dichroic mirror: 785 nm
Image Splitting Dichroic
- DM A561 nm long pass (FITC / TRITC-dual camera)
- Glass (transmits to camera 2)
- Mirror (reflects to camera 1)
Emission filter wheel camera 1 (Photometrics BSI)
- quad-band 440/521/607/700 nm → DAPI / FITC / TRITC / Cy5
- single bandpass: 447/60 nm → DAPI / BFP
- single bandpass: 525/30 nm → GFP, Venus, mNeonGreen, Alexa488, FITC
- quad-band 440/521/607/700 nm → DAPI / FITC / TRITC / Cy5
- single bandpass: 447/60 nm → DAPI / BFP
- single bandpass: 525/30 nm → GFP, Venus, mNeonGreen, Alexa488, FITC
- single bandpass: 600/52 nm → TRITC, mCherry
- single bandpass: 708/75 nm → Cy5
- single bandpass: 835/70 nm → NIR
Cameras: 2 x Teledyne Photometrics BSI (USB3) sCMOS camera, 95% quantum efficiency; 6.5 µm x 6.5 µm pixel area
Computer (HP Z8 Workstation):
Dual Intel Xeon 4114 2.2 2400 MHz 10 CPU
RAM: 128 GB DDR4 2666 MHz
Nvidia Quadro RTX5000
2 TB SSD drive for fast imaging
6 TB HDD
10 GB Ethernet card
Contact: