Widefield fluorescent microscope with incubation chamber and CO2 mixer available
Location: i06.U1.406
Specifications:
- Timelapse option, motorized stage
- Perfect Focus System (PFS)
- DS-Qi2 monochrome camera
- DIC imaging with 10x, 20x and 40x objectives
- Nikon JOBS software: automation of acquisition
Objectives:
- Plan Fluor 4x/0.2 air
- Plan Fluor 10x/0.3 DIC 1 N1 air PFS
- Plan Apo λ 20x/0.75 DIC 1 air PFS
- Plan Apo λ 40x/0.95 DIC air PFS
Emission filters
-
- DAPI (LP 409, BP 447/60)
- GFP (LP 495, BP 520/35)
- mCherry (LP 596, BP 641/75)
- FarRed – Cy5 (LP 669, BP 692/40)
- TripleBand Spectra X (DAPI,GFP, mCherry)
- CFP (LP 458, BP 483/32) – available on request, contact BIF 24h prior to imaging time slot
Lumencor light source with flexible excitation band selection:
-
- Violet (390/18)
- Blue (438/24)
- Cyan (475/28)
- Teal (513/17)
- Green (549/15)
- Yellow (575/25)
- Red (632/22)
System manual and additional material is available on SeaFile (login required).
Contact: Imaging & Optics Facility
Resources:
To open ND2 files in FIJI: go to Plugins > Bio-Formats > Bio-Formats Plugins Configuration. Under the tab Formats find Nikon ND2 (it is sorted alphabetically) and disable Use chunkmap table.
If this does not work, try enabling the Nikon reader or different combinations of the last 2 check boxes.