Zeiss Lightsheet 7

Neurons from a cleared mice brain, imaged using ZEISS LS7 5x/0.16 foc objective.

 

Zebrafish heart beat at ~130 frames/s

Location: I06.U1.405

Light sheet microscope with vertical mounting,climate controlled chamber ( humidity – CO₂ and O₂ control). The different sample chambers available for the Lightsheet 7makes imaging of living model organisms, cleared and expanded samples possible. The adaptive optics allow to adjust for different refractive indices (RI)

---

Specification

• Chamber Climate Control (Humidity – CO₂ and O₂ control).
• 6 laser lines
• Dual side illumination
• Automated axis XYZ+ Rotation
• Multiview (angles) acquisition
  Multiview Acquisition quick setup
• Pivot scan (reducing shadows)
• Multi-position & tiling
• Additional piezo driven z-axis for fast imaging
• Dual camera (PCO edge 4.2 sCMOS)
• Versatile chambers and sample holders
  

---

Objectives

Illumination objectives

• 5x/0.1foc (for 5x or 10x detection objective)
• 10x/0.2foc (for 20x detection objectives)

Detection objectives

Water immersion:

• 5x/0.16: EC-Plan Neofluar nd=1 (WD= 5.1 mm)
• 10x/0.5: W Plan-Apochromat nd=1,33 (WD=3.7 m)
• 20x/1.0: W Plan-Apochromat Corr DIC; nd=1.33 (WD=2.4 mm)

Cleared samples:

• 5x/0.16: foc EC-Plan Neofluar nd=1 (WD= 10.5 mm)
• 20x/1.0: Clr Plan-Apochromat Corr nd=1.38 (WD=5.6 mm)
• 20x/1.0: Clr Plan-Neofluar Corr nd=1.45 (WD=5.6 m)
• 20x/1.53: Clr Plan-Neofluar Corr DIC; nd=1.53 (WD=  6.4mm)
  
  

  Objective lens	Free working distance	Smallest pixel size ->camera Pixel size / max zoom	Optical resolution @500nm (0.61x500nm/NA)	Refractive index
  EC Plan-NEOFLUAR 5x/0,16	10.5 mm	520 nm	1906 nm	Adaptive
  W Plan-Apochromat 10x/0.5	3.7 mm	260 nm	610 nm	1.33
  W Plan-Apochromat 20x/1,0 DIC	2.4 mm	130 nm	305 nm	1.33
  Clr Plan-Neofluar 20x/1.0 Corr nd=1.38	5.6 mm	130 nm	305 nm	1.38
  Clr Plan-Neofluar 20x/1.0 Corr nd=1.45	5.6 mm	130 nm	305 nm	1.45
  Clr Plan-Neofluar 20x/1,0 Korr nd=1,53	6.4 mm	130 nm	305 nm	1.53

Refractive index of some solutions:

---

Sample Chambers

• Chamber #1: Water / Live 20x → Fixed / Live (temperature; CO2, O2, humidity -control)
• Chamber #2: Cleared Sample 20x (n=1.35-1.58)
• Chamber #3: Large Sample 5x (n=1.33-1.58)

---

Laser lines

• 405nm / 50 mW
• 445nm / 24 mW
• 488nm / 50mW
• 515nm / 20 mW
• 561nm / 50mW
• 638nm / 75mW
  

---

Filters / Beam-splitters

Filter wheel 1:

• DAPI–GFP: BP 420-470/ LP 490/ BP 505-545
• GFP– CY3: BP 505-545/LP560/ BP 575-615
• GFP– DRAQ5: BP 505-545/LP560/LP660
• GFP (narrow)-mCherry: BP 505-530/LP560/LP585
• GFP–DRAQ5: BP 505-545/LP560/LP660

Filter wheel 2:

• DAPI–GFP: BP 420-470/ LP 490/ BP 505-545
• CFP– YFP: BP 460-500/LP510/ BP 525-562
• CFP– CY3: BP 460-500/LP510/LP 575-615
• CFP– mCherry: BP 460-500/LP510/LP585
• GFP–DRAQ5: BP 505-545/LP560/LP660

---

Resources:

– System manual and additional material is available on SeaFile (login required).

– How to Get Best Images with Various Types of Immersion Media and Clearing Agents

– IOF Sample Clearing page

---