Zeiss LSM-08 (880inv_Airyscan)

Confocal scanner head for 3 descanned spectral channels

Location: room I06.U1.406

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specification: Zeiss Axio Observer Z1inverted microscope from Zeiss (Germany)

  1. one GaAsP PMT
  2. two multialkali PMTs
  3. A transmitted light (T-PMT) detector.
  4. An AiryScan super-resolution detector with Fast Airyscan option.

Software & Modules: ZEN black edition 2.3 SP1 + ZEN blue edition 2.3 (for image processing only!!!)

  1. Experiment designer
  2. Airyscan
  3. FCS

Illumination / excitation:

  1. Argon laser lines: 458nm, 488nm, 514nm
  2. Violet (405nm)
  3. green (561nm) and red (633nm) Diode lasers
  4. pE-300 Lite fluorescent lamp  (visual eximination)
  5. Halogen (visual eximination)

Sample conditioning:

  1. Temperature chamber
  2. Gas Incubation system for CO2 + O2 available from Ibidi (Germany)

Objectives:

Pos.1 Plan-Apochromat 10x / NA 0.45 Air (WD=2.1mm)

Pos.2 Plan-Apochromat 20x / NA 0.8 Air

Pos.3 Plan-Apochromat 40x / NA 1.3 OIL

Pos.4 Plan-Apochromat 63x / NA 1.4 Oil

Pos.5 LD C-Apochromat 40x / NA 1.1 Water

Pos.6 C-Apochromat 63x / NA 1.2 Water

Modalities

  1. Multiple position imaging

PC: HP Z820 workstation

  • Intel® Xeon® CPU E5-2643 0 @ 3.3GHz
  • 80GB RAM
  • 1.81TB data drive

Contact persons:

System manual and additional material is available on SeaFile (login required).

Booking: system in PMS SSU portal  

Laser safety: Document in PMS SSU portal