UV laser ablation & micro-dissection

 

UV laser ablation can be used to quantitatively determine mechanical tension in tissues and cultured cells, but also to do selective ablation of cells or structures. The UV laser ablation functionality is build on the Andor CSU-X1 Spinning Disk Confocal Microscope and can also be used for micro-dissection.

More information: Smutny M., Behrndt M., Campinho P., Ruprecht V., Heisenberg CP. (2015) UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo In Vivo and Ex Vivo. In: Nelson C. (eds) Tissue Morphogenesis. Methods in Molecular Biology (Methods and Protocols), vol 1189. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1164-6_15

https://link.springer.com/protocol/10.1007/978-1-4939-1164-6_15