Location:
Room i04.UG.015 –
Imaging modalities/features:
- Confocal + real-time deconvolution (Lightning)
- time gated acquisition
- life time imaging
- spectral unmixing
- excitation Λ / emission λ wavelength scans
Specification:
- DM6 CS 5 Research microscope
- TCS SP8 FALCON
- Motorised Scanning stage
Light sources:
- Lumencor SOLA SM II, LED Light source
- 405 nm laser: DMOD (50 mW) – Continuous wave
- White Lite Laser (470-670 nm) Supercontinuum pulsed laser (max. 80 MHz): tunable in steps of 1 nm – allows simultaneous use of up to 8 laser lines from UV to IR (8 channel AOTF: rapid modulation of laser intensity, particularly for ROI)
Filter for visual examination:
- DAPI: Excitation: BP 360/40 Emission: BP 470/40
- FITC: Excitation: BP 470/40 Emission: BP 525/50
- RHOD: Excitation: BP 546/10x Emission: BP 585/40
Objectives – positions:
- HC PL Fluotar 5x / 0.15 (# 15506224), WD 12.0 mm – no immersion
- –
- HC FUOTAR L 25x / 0.95 W (# 15506374), WD=2.5 mm, wide angle (41°)
- –
EXTRA high-end objectives are available via Automated Rental System:
- HC PL APO 20x / 0.75 MI (# 15506343), WD 0.67 mm – multi-immersion (CS2): water, glycerine and immersion oil
Detection Filters:
- NF 445
- NF 488
- NF 514
- NF 458/514
- NF 488/561/633
- NF 594
- NF 445/594
Detectors: 4 channel SP + BF
- PMT
- HyD SP GaAsPDetektor for gated imaging
- PMT
- HyD2 SMD: specially selected for Falcon FLIM)
- Transmitted light detector (TLD)
Computer (CUDA Workstation):
- Intel 6134 Xeon 3.2 8C
- 192 GByte RAM
- NVIDIA Quadro GP100 16GB Reference GPU (3584 cores)
- 256 GB SATA SSD
- 6 TByte SATA hard disk drive
- 2 TB SSD RAID 0
- 10Gb network card
Modules:
- LAS X Live Data Mode
- LAS X Dye Finder
- LAS X 3D Visualisation
- LAS X Phasor FLIM online
- LAS X LIGHTNING Expert: Confocal SuperResolution
System manual and additional material is available on SeaFile (login required).
Contact: