Zeiss Lattice Lightsheet 7

Location: I06.U1.405

The Lattice Light Sheet Microscope (LLSM) is designed for long-term volumetric imaging at sub-cellular resolution. The system allows standard sample carriers, and is equipped with temperature, humidity and CO2 control.  The Lattice Lightsheet technologies reduces phototoxicity and photobleatching, allowing monitoring of cellular processes for extended period (hours – to – days).

 

when to choose the 'Lattice Lightsheet' over the 'Gaussian Lightsheet'

The Lattice Light Sheet Microscope takes over where there solution of the Gaussian light sheet (see our Zeiss Lightsheet 7) comes to its limits for sub-cellular imaging. The figure below gives an impression of the feasible size ranges: your samples should not exceed the range limit of maximum 300 µm in XY and 200 µm in Z. Samples can be mounted on well plates, Mattek dishes and slides (cover glass no. 1.5).

Advantages of LLSM

  • Very fast
  • Gentle illumination of the cells and fluorophores
  • Gives enhanced resolution in the confocal range
  • Inverse configuration allows the use of standard sample carriers
  • Sample alignment and leveling is performed automatically by the system
  • Automated immersion media supply (water)

The Zeiss Lightsheet 7 is fully equipped for larger sample imaging, that include organoids, model organisms, cleared and expanded tissues.

Specification

  • Multi-position & Tiling
  • Versatile chambers and sample holders, with temperature, humidity and CO2 control. 
  • Additional piezo driven z-axis for fast imaging
  • Transmitted Light (+ enhanced oblique contrast)
  • Dual camera (2 x Hamamatus Orca Fusion sCMOS)
  • Lattice Light sheets (typical): 500nm x 12µm ;650nm x 15µm; 750nm x 30µm; 1000nm x 30µm;1400nm x 85µm; 2000nm x 100µm
  • Lateral resolution: 330nm x 330nm (+ Deconvolution: 290nm x 290n)
  • Axial resolution(dependingon lightsheet): ranging from500nm to1000nm (+ Deconvolution: ranging
    from450nm and 900nm)

Objectives

The system is equipped with dedicated optics, and an automated water-immersion dispenser

  1. Excitation objective: 10x / 0.4, Water Immersion
  2. Meniscus lens
  3. detection objective: 48x / 1.0, Water Immersion + motorized coverslip adjustment

Schematic below show the sample carrier and core optics module with excitation objective (1), meniscus lens (2) and detection objective with free-form optics (3).

Sample mounting

The system is optimized for conventional sample preparation techniques, and is quipped with temperature; CO2, O2, humidity -control.   Please take not to use the correct cover slips (#1.5) !

  • holder #1: pertridish 35-40mm
  • holder #2: chamber slides 25 x 57mm
  • holder #2: multiwell plates 85 x 127 mm

Laser lines

The Lattice Light Sheet 7 is configured with the “high-laserpower laser module” to allow more flexibility and explore SMLM applications

  • 488nm (14mW)
  • 561nm (9mW)
  • 638nm (13mW)

Filters / Beam-splitters

Dichroic / Camera Splitter:

  1. LP 565
  2. LP 640

Camera Filters:

position

Camera 1: direct light path

Camera 2: reflected light path

1

BP 570-620 + LP655

BP 570-610 IR+

2

BP 495-550 + LP655

empty

3

BP 495-550  /  BP 570-620

BP 495-550 / BP 570-620

4

LBF 405/488/561/642

BP 500-550 IR+

5

1% ND

  

6

empty

  

7

BP 495-590

  

8

LP 570

  

 

Camera’s

  • 2x Hamamatsu ORCA-Fusion sCMOS (> 82% QE und 6.5 um Pixelsize)

Resources: