Zeiss Lightsheet 7

Posted onAuthorGabriel Krens

Zeiss Lightsheet 7
Zeiss Lightsheet 7 - Image 2
Zeiss Lightsheet 7 - Image 3
Zeiss Lightsheet 7 - Image 4
Zeiss Lightsheet 7 - Image 5

Light sheet microscope with vertical mounting,climate controlled chamber ( humidity – CO2 and O2 control). The different sample chambers available for the Lightsheet 7makes imaging of living model organisms, cleared and expanded samples possible. The adaptive optics allow to adjust for different refractive indices (RI)

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Description

Location: I06.U1.405

Specification

Objectives

Illumination objectives

  • 5x/0.1foc (for 5x or 10x detection objective)
  • 10x/0.2foc (for 20x detection objectives)

Detection objectives

Water immersion:

  • 5x/0.16: EC-Plan Neofluar nd=1 (WD= 5.1 mm)
  • 10x/0.5: W Plan-Apochromat nd=1,33 (WD=3.7 m)
  • 20x/1.0: W Plan-Apochromat Corr DIC; nd=1.33 (WD=2.4 mm)

Cleared samples:

  • 5x/0.16: foc EC-Plan Neofluar nd=1 (WD= 10.5 mm)
  • 20x/1.0-nd=1.38: Clr Plan-Apochromat Corr nd(correction range)=1.33-1.38 (WD=5.6 mm)
  • 20x/1.0-nd=1.45: Clr Plan-Neofluar Corr nd(correction range)=1.38-1.45 (WD=5.6 m)
  • 20x/1.0-nd=1.53: Clr Plan-Neofluar Corr DIC; nd(correction range)=1.45-1.53 (WD=  6.4mm)

Objectives

Objective lens Free working distance Smallest pixel size ->camera Pixel size / max zoom Optical resolution @500nm (0.61x500nm/NA) Refractive index
EC Plan-NEOFLUAR 5x/0,16 10.5 mm 520 nm 1906 nm Adaptive
W Plan-Apochromat 10x/0.5 3.7 mm 260 nm 610 nm 1.33
W Plan-Apochromat 20x/1,0 DIC 2.4 mm 130 nm 305 nm 1.33
Clr Plan-Neofluar 20x/1.0 Corr nd=1.38 5.6 mm 130 nm 305 nm 1.38
Clr Plan-Neofluar 20x/1.0 Corr nd=1.45 5.6 mm 130 nm 305 nm 1.45
Clr Plan-Neofluar 20x/1,0 Korr nd=1,53 6.4 mm 130 nm 305 nm 1.53

Sample Chambers

  • Chamber #1: Water / Live 20x → Fixed / Live (temperature; CO2, O2, humidity -control)
  • Chamber #2: Cleared Sample 20x (n=1.35-1.58)
  • Chamber #3: Large Sample 5x (n=1.33-1.58)

Refractive Indexes - to help to select matching objectives & sample chambers

Laser lines

  • 405nm / 50 mW
  • 445nm / 24 mW
  • 488nm / 50mW
  • 515nm / 20 mW
  • 561nm / 50mW
  • 638nm / 75mW

Filters / Beam-splitters

Laser blocking filter1 (LBF1):

405 nm,488 nm,561 nm,640 nm

Laser blocking filter2 (LBF2):

445 nm, 561 nm, 640 nm

Filter wheel 1:

  • DAPI-GFP: BP 420-470/ LP 490/ BP 505-545
  • CFP- CY3: BP 420-470/LP490/ BP 575-615
  • GFP- DRAQ5: BP 505-545/LP560/LP660
  • GFP (narrow)-mCherry: BP 505-530/LP560/LP585
  • GFP-DRAQ5: BP 505-545/LP560/LP660

Filter wheel 2:

  • DAPI-GFP: BP 420-470/ LP 490/ BP 505-545
  • CFP- YFP: BP 460-500/LP510/ BP 525-562
  • CFP- CY3: BP 460-500/LP510/LP 575-615
  • CFP- mCherry: BP 460-500/LP510/LP585
  • GFP-DRAQ5: BP 505-545/LP560/LP660

Resources:

– System manual and additional material is available on SeaFile (login required).

– How to Get Best Images with Various Types of Immersion Media and Clearing Agents

– IOF Sample Clearing page

Additional information

Building

i06
Camera / Detector

sCMOS
Fluorescent Lightsource

Laser: 405±5 nm, Laser: 445±5nm, Laser: 488±5 nm, Laser: 514±5nm, Laser: 561±5 nm, Laser: 640±5 nm
System_Specification

Illumination: Fluorescence, Illumination: Bightfield, Incubation: heating chamber, Incubation: heating stage, Incubation: CO2/N2-gas mixer (hypoxia), Automated Stage, Dual Camera (see detectors)
Technology / Application

Time-lapse recording, Multi-position, In-vivo imaging

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