Description
Location: I06.U1.405
Specification
Objectives
Illumination objectives
- 5x/0.1foc (for 5x or 10x detection objective)
- 10x/0.2foc (for 20x detection objectives)
Detection objectives
Water immersion:
- 5x/0.16: EC-Plan Neofluar nd=1 (WD= 5.1 mm)
- 10x/0.5: W Plan-Apochromat nd=1,33 (WD=3.7 m)
- 20x/1.0: W Plan-Apochromat Corr DIC; nd=1.33 (WD=2.4 mm)
Cleared samples:
- 5x/0.16: foc EC-Plan Neofluar nd=1 (WD= 10.5 mm)
- 20x/1.0-nd=1.38: Clr Plan-Apochromat Corr nd(correction range)=1.33-1.38 (WD=5.6 mm)
- 20x/1.0-nd=1.45: Clr Plan-Neofluar Corr nd(correction range)=1.38-1.45 (WD=5.6 m)
- 20x/1.0-nd=1.53: Clr Plan-Neofluar Corr DIC; nd(correction range)=1.45-1.53 (WD= 6.4mm)
Objectives
| Objective lens |
Free working distance |
Smallest pixel size ->camera Pixel size / max zoom |
Optical resolution @500nm (0.61x500nm/NA) |
Refractive index |
Mounting solutions compatibility (YES, NO) |
| EC Plan-NEOFLUAR 5x/0,16 |
10.5 mm |
520 nm |
1906 nm |
Adaptive |
Water, E3, 1xPBS, Cubic, EZ index, oil (Mineral + PDM only -> replacing CUBIC or EZ index) , DABB |
| W Plan-Apochromat 10x/0.5 |
3.7 mm |
260 nm |
610 nm |
1.33 |
Water, E3, 1xPBS, Cubic, EZ index, oil (Mineral+PDM only-> replacing CUBIC or EZ index), DABB |
| W Plan-Apochromat 20x/1,0 DIC |
2.4 mm |
130 nm |
305 nm |
1.33 |
Water, E3, 1xPBS, Cubic, EZ index, oil (Mineral+PDM only-> replacing CUBIC or EZ index), DABB |
| Clr Plan-Neofluar 20x/1.0 Corr nd=1.38 |
5.6 mm |
130 nm |
305 nm |
1.38 |
Water, E3, 1xPBS, Cubic, EZ index, oil (Mineral+PDM only-> replacing CUBIC or EZ index), DABB |
| Clr Plan-Neofluar 20x/1.0 Corr nd=1.45 |
5.6 mm |
130 nm |
305 nm |
1.45 |
Water, E3, 1xPBS, Cubic, EZ index, oil (Mineral+PDM only-> replacing CUBIC or EZ index), DABB |
| Clr Plan-Neofluar 20x/1,0 Korr nd=1,53 |
6.4 mm |
130 nm |
305 nm |
1.53 |
Water, E3, 1xPBS, Cubic, EZ index, oil (Mineral+PDM only-> replacing CUBIC or EZ index), DABB |
Sample Chambers
- Chamber #1: Water / Live 20x → Fixed / Live (temperature; CO2, O2, humidity -control)
- Chamber #2: Cleared Sample 20x (n=1.35-1.58)
- Chamber #3: Large Sample 5x (n=1.33-1.58)
Refractive Indexes - to help to select matching objectives & sample chambers
Laser lines
- 405nm / 50 mW
- 445nm / 24 mW
- 488nm / 50mW
- 515nm / 20 mW
- 561nm / 50mW
- 638nm / 75mW
Filters / Beam-splitters
Laser blocking filter1 (LBF1):
405 nm,488 nm,561 nm,640 nm
Laser blocking filter2 (LBF2):
445 nm, 561 nm, 640 nm
Filter wheel 1:
- DAPI-GFP: BP 420-470/ LP 490/ BP 505-545
- CFP- CY3: BP 420-470/LP490/ BP 575-615
- GFP- DRAQ5: BP 505-545/LP560/LP660
- GFP (narrow)-mCherry: BP 505-530/LP560/LP585
- GFP-DRAQ5: BP 505-545/LP560/LP660
Filter wheel 2:
- DAPI-GFP: BP 420-470/ LP 490/ BP 505-545
- CFP- YFP: BP 460-500/LP510/ BP 525-562
- CFP- CY3: BP 460-500/LP510/LP 575-615
- CFP- mCherry: BP 460-500/LP510/LP585
- GFP-DRAQ5: BP 505-545/LP560/LP660
Resources:
– System manual and additional material is available on SeaFile (login required).
– How to Get Best Images with Various Types of Immersion Media and Clearing Agents
– IOF Sample Clearing page
