Zeiss LSM-04 (800-inverted)

Description

This confocal microscope with Airyscan: well suited for life imaging: 

Axio Observer.Z1, Inverted LSM800 confocal microscope + Airyscan, suited for live imaging: equipped with heating chamber, CO₂ mixer,  ZEN 3.8 with experiment designer,macro-enviroment (OAD) and ZEN Connect.

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Sample conditioning:

• Heating chamber
• CO₂ mixer + humidifier

Light sources:

• Laser lines 405, 488, 561, 640 nm
• CoolLED p300 SB light
• ESID LED

Detectors: 3 channels simultaneously (more sequentially)

• 2x GaAsP PMTs: Gallium arsenide phosphide photomultiplier tube detectors with free choice of the spectral range. (Uses a tuneable short-pass and band-pass filter in front of the PMTs)
• 1 x Airyscan detector – optimized for 63x. Can also be used as 3rd GaAsP detector!
• 1x ESID+LED light source: Electronically switchable illumination and detection module

Motorized stage for multiple position imaging

• Motorized stage for multiple position imaging
• Multi-slide holder, dish holder, single slide holder

Objectives (M27 thread):

1. Plan-Apochromat 10x/0.45, WD=2.1 mm (420640-9900-000)
2. Plan APOCHROMAT 20x/0.8 , WD=0.55 mm (D=0.17 mm) (420650-9901)
3. Plan-Apochromat 40x/1.2 Water
4. Plan-APOCHROMAT 63x/1.4 Oil (420782-9900-799)
5. Plan-Apochromat 100x / NA 1.4 Oil  DIC

Software: Zen 3.8

1. ZEN Module Experiment Designer Hardware
2. ZEN Module Tiles & Positions
3. ZEN Macro Editor

Computer: HP Z6

• Intel® Xeon™ Gold 5122 v3 CPU @ 3.6GHz + 3.59 GHz
• 64GB RAM
• NVIDIA Quadro P400 (8Gb)
• 8 TB data drive

Contacts person:

• iof@ist.ac.at

System manual and additional material is available on SeaFile (login required).