Axio Observer.Z1 Inverted LSM900 confocal microscope, suited for live imaging: equipped with heating chamber, CO2 mixer, ZEN 3.8 with experiment designer. This confocal microscope is well suited for live imaging book in PPMS
Description
Location: i06.U1.403
Axio Observer.Z1 Inverted LSM900 confocal microscope, suited for live imaging: equipped with heating chamber, CO2 mixer, ZEN 3.8 with experiment designer. This confocal microscope is well suited for live imaging book in PPMS
Sample conditioning:
- Heating chamber
- CO2 mixer available
Light sources:
- Laser lines 405, 488, 561, 640 nm
- CoolLED p300 SB light
- ESID LED
Detectors: 3 channels simultaneously (more sequentially)
- 3x GaAsP PMTs: Gallium arsenide phosphide photomultiplier tube detectors with free choice of the spectral range. (Uses a tunable short-pass and band-pass filter in front of the PMTs)
- 1x ESID+LED light source: Electronically switchable illumination and detection module
Motorized stage for multiple position imaging
- Multi-slide holder
- Dish holder
- Single slide holder
Objectives (M27 thread):
- Plan-Apochromat 10x/0.45 (420640-9900), WD=2.1 mm
- Plan APOCHROMAT 20x/0.8 (420650-9901), WD=0.55 mm (D=0.17 mm)
- Plan-Apochromat 40x/1.2 Water, WD=0.28 mm
- –
- Plan-APOCHROMAT 40x/1.4 Oil, DIC, WD=0.13
- empty
Software: Zen 3.8
- ZEN Module Experiment Designer Hardware
- ZEN Module Tiles & Positions
Computer: PC Esprimo P920
- Intel® Core™ i5-4670 CPU @ 3.4GHz
- 32GB RAM
- 1.81TB data drive
Contact persons:
- iof@ist.ac.at
System manual and additional material is available on SeaFile (login required).
Additional information
Building | i06 |
---|---|
Camera / Detector | GaAsP-PMT, ESID |
Fluorescent Lightsource | Laser: 405±5 nm, Laser: 488±5 nm, Laser: 561±5 nm, LED: 355-365nm, LED: 430-450nm, LED: 545-555nm, LED: 640-650nm |
System_Specification | Illumination: Fluorescence, Incubation: heating chamber, Incubation: CO2-gas mixer, Automated Stage |
Technology / Application | FRAP, FRET, Time-lapse recording, Multi-position, In-vivo imaging, Photoactivation / -conversion |