Zeiss LSM-03 (910-inverted)

Description

Location: I06.U1.403

Inverted Confocal microscope, ZEN 3.12 with experiment designer and ZEN Connect.

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Specifications:

• Axio Observer.Z1, Inverted Confocal microscope, ZEN 3.12 with experiment designer.
• 2x (MA-PMT) channels for simultaneously imaging
• motorized stage: Multiple position imaging, tilescan
• Automation: Timelapse, Experiment designer,
• Zen Connect: multi-modal combination of different Zeiss machines

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Light sources:

• Laser lines 405, 488, 561, 640 nm
• Zeiss Viluma 7-lines LED: 385/30; 423/44; 467/38; 508/20; 555/30; 591/27; 631/33; 735/40 nm
• ESID LED

Detectors: 3 channels simultaneously; Airyscan; Lightfield 4D

• 2x MA-PMT: Multi Alkali Photomultiplier Tube detectors with free choice of the spectral range. (Uses a tunable short-pass and band-pass filter in front of the PMTs)
• 1x Airyscan II
• 1x Lightfield 4d
• 1x ESID+LED light source: Electronically switchable illumination and detection module

Motorized stage for multiple position imaging

• Motorized stage for multiple position imaging
• Multi-slide holder, dish holder, single slide holder

Objectives (M27 thread)

1. Plan-Apochromat 5x (Calibration objective-not mounted)
2. Plan-Apochromat 10x/0.45
3. Plan Apochromat 20x/0.8
4. Plan-Apochromat 25x/0.8 Immersion
5. Plan-Apochromat 40x/1.2 Water
6. Plan-Apochromat 63x/1.4 oil

Software: Zen 3.12

1. ZEN Module Experiment Designer Hardware
2. ZEN Module Tiles & Positions

Computer:

• Intel® Xeon® w5-3425 Processor (30M Cache, 3.20 GHz)
• 64GB RAM
• 3.38TB data drive

System manual and additional material is available on SeaFile (login required).