Light sheet microscope with vertical mounting,climate controlled chamber ( humidity – CO2 and O2 control). The different sample chambers available for the Lightsheet 7makes imaging of living model organisms, cleared and expanded samples possible. The adaptive optics allow to adjust for different refractive indices (RI)
Description
Location: I06.U1.405
Specification
- Chamber Climate Control (Humidity – CO2 and O2 control).
- 6 laser lines
- Dual side illumination
- Automated axis XYZ+ Rotation
- Multiview (angles) acquisition
Multiview Acquisition quick setup - Pivot scan (reducing shadows)
- Multi-position & tiling
- Additional piezo driven z-axis for fast imaging
- Dual camera (PCO edge 4.2 sCMOS)
- Versatile chambers and sample holders
| Objective lens | Free working distance | Smallest pixel size ->camera Pixel size / max zoom | Optical resolution @500nm (0.61x500nm/NA) | Refractive index |
| EC Plan-NEOFLUAR 5x/0,16 | 10.5 mm | 520 nm | 1906 nm | Adaptive |
| W Plan-Apochromat 10x/0.5 | 3.7 mm | 260 nm | 610 nm | 1.33 |
| W Plan-Apochromat 20x/1,0 DIC | 2.4 mm | 130 nm | 305 nm | 1.33 |
| Clr Plan-Neofluar 20x/1.0 Corr nd=1.38 | 5.6 mm | 130 nm | 305 nm | 1.38 |
| Clr Plan-Neofluar 20x/1.0 Corr nd=1.45 | 5.6 mm | 130 nm | 305 nm | 1.45 |
| Clr Plan-Neofluar 20x/1,0 Korr nd=1,53 | 6.4 mm | 130 nm | 305 nm | 1.53 |
Resources:
– System manual and additional material is available on SeaFile (login required).
– How to Get Best Images with Various Types of Immersion Media and Clearing Agents
Additional information
| Building | i06 |
|---|---|
| Camera / Detector | sCMOS |
| Fluorescent Lightsource | Laser: 405±5 nm, Laser: 488±5 nm, Laser: 561±5 nm, Laser: 640±5 nm |
| System_Specification | Illumination: Fluorescence, Illumination: Bightfield, Incubation: heating chamber, Incubation: heating stage, Incubation: CO2/N2-gas mixer (hypoxia), Automated Stage, Dual Camera (see detectors) |
| Technology / Application | Time-lapse recording, Multi-position, In-vivo imaging |
