The vertical point scanning confocal microscope and root-tracker is a unique device that allows to image and track multiple samples / plant roots over a prolonged time in an upright position
Description
Location: I04.O3.014
System description: Vertical confocal microscope
- 2 MA-PMT detectors: 2 channels simultaneously, more sequentially
- Root-tracker (2 versions: Zeiss OAD macro or Matlab script)
- Multiple position imaging
- Gravitation manipulation (rotation stage)
Laser lines: 405; 488; 561; 640 nm
Objectives (M27 thread)
- Plan-Apochromat 10x/0.45 (420640-9900), WD=2.1 mm
- Plan APOCHROMAT 20x/0.8 (420650-9901), WD=0.55 mm (D=0.17 mm)
- Plan-Apochromat 40x/1.2 Water (D=0.13-0.217 mm)
- EC-Plan-NeoFluar 2.5x /0.075 Dry
- C-Apochromat 40x/0.95 Dry (optional, accessible via the rental system)
Contact: email
System manual and additional material is available on SeaFile (login required).
- FIJI script to convert the image-sequence from tracker to concatenated files per position can be found here
- More details about the system can be found here: https://elifesciences.org/articles/26792
I am the one on the right (LSM800-05)
Additional information
| Building | i04 |
|---|---|
| Camera / Detector | MA-PMT, ESID |
| Fluorescent Lightsource | Laser: 405±5 nm, Laser: 488±5 nm, Laser: 561±5 nm, Laser: 640±5 nm, LED: 355-365nm, LED: 430-450nm, LED: 545-555nm, LED: 640-650nm |
| System_Specification | Illumination: Fluorescence, Illumination: Bightfield, Automated Stage, Microfluidics |
| Technology / Application | FRAP, FRET, Time-lapse recording, Multi-position, Sample Stabilization / (Root-)Tracker, In-vivo imaging, Photoactivation / -conversion |
