The Imaging & Optics Facility provides a full slide-scanner service → details Slide Scanner Service
The Imaging & Optics Facility provides a full Image Acquisition Service for Slide-Scanning. One main advantages for using this service is that the acquisition is performed by Imaging and optics Facility staff – which can save time for your other research tasks. The service offers:
- Option to choose and combine different imaging modality (brightfield or fluorescence)
- Generation of overview scans for fast screening and sample navigation (see point 6)
- 2D or 3D imaging capabilities.
- Include high-resolution imaging of selected Regions Of Interest (ROIs), including shading correction & stitching
- Optional: scene splitting, further post processing and image analysis routine: deconvolution, segmentation
- Scanned images can be used for navigation on LSM confocal microscopes via ZEN Connect
Description
Location: I06.U1.405
Specification:
- 25 mounting frames for 4 slides (= 100 slides loading capacity in 1 time)
- Bright field imaging
- Fluorescent
Contrast techniques:
- TL Brightfield
- TL Ring Aperature Contrast (RAC)
Light sourse: 7 solid state LED lamps (Kolibri 7)
- Red (630nm) for excitation of Cy5, Alexa 631, TOTO-3 and similar dyes
- Yellow (590nm) for excitation of mCherry, Alexa 568, mPlum and similar dyes
- Green (555nm) for excitation of Cy3, TRITC, DsRed and similar dyes
- Cyan (511nm) for excitation of eYFP, Eosin, TOTO-1 and similar dyes
- Blue (475nm) for excitation of eGFP, Fluo4, FITC and similar dyes
- Violet (430nm) for excitation of eCFP, Lucifer Yellow, Alexa 430 and similar dyes
- UV (385nm) for excitation of DAPI, Alexa 405, Hoechst 33258 and similar dyes
Objectives:
- Fluar 5x/0.25 M27 (FWD=12.5mm)
- Plan-Apochromat 10x/0.45 M27 (FWD=2.1mm)
- Plan-Apochromat 20x/0.8 M27
- Plan-Apochromat 40x/0.95 Corr M27 (FWD=0.25mm)
Cameras:
- Color camera: Hitachi HV F202 (Histology)
- Monochrome: Hamamatsu Orca Flash 4.0 V3 (Fluorescence)
Modules:
- ZEN Connect
- ZEN Module Macro Environment
- ZEN Module Image Analysis
- ZEN Module Advanced Processing & Analysis
- ZEN Module Intellesis
Additional information
| Building | i06 |
|---|---|
| Camera / Detector | Color CMOS, sCMOS |
| Fluorescent Lightsource | LED: 385-395nm, LED: 430-450nm, LED: 470-475nm, LED: 505-515nm, LED: 545-555nm, LED: 640-650nm |
| System_Specification | Illumination: Fluorescence, Illumination: Bightfield, Automated Stage, Focus Stabilization |
| Technology / Application | Multi-position, High-troughput Screening |
