The Imaging and Optics Facility (IOF) is developing continuously. On this page we provide an overview of the ongoing activities in our events calendar (current maintenance, workshops, equipment demonstrations, courses,.. ), and link to the archive of our weekly update newsletter. to previous teaching activities (courses & seminars) and equipment demonstration & workshops.
Events
- i24/Moonstone: Maintenance weeks will be from September 8 until October 3rd. C&M will inform us 2 weeks before the maintenance weeks start when and how (electricity, ventilation) our Moonstone facility will be affected. You will get detailed information as soon as we have it in the weekly update and see it as incidents in PPMS.
Abberior Mirava Polyscope workshop on 15-19 September
This system unites confocal, STED and Single Molecule imaging techniques to cover a wide range of resolution, extending over several orders of magnitude from diffraction-limited imaging all the way down to true molecular resolution.
Additional features: FLIM imaging; Matrix detector for background removal, Adaptive Optics for image optimization of thick samples
Monday 15 September 10 am @ Mondi 3 : introduction to Mirava Polyscope by Steffen Restel (Abberior application specialist)
ZOOM: https://istaustria.zoom.us/j/63521800576?pwd=b0cJ2atbBhfy6LqAixmhjq2SlaZY9U.1
Tuesday 16 September: IOF internal software training
Wednesday 17 September: Live cell staining and STED imaging: open for all workshop participants !!!
| Individual imaging sessions | Thursday 18 September | Friday 19 September |
| 9:30 – 11:30 | occupied | occupied |
| 12:30 – 14:30 | occupied | occupied |
| 15:00 – 17:00 | occupied | xxx |
We are happy to announce the seminar “Introduction to Mirava Polyscope” by Steffen Restel (Abberior application specialist), on Monday 15 September 10 am @ Mondi 3
ZOOM: https://istaustria.zoom.us/j/63521800576?pwd=b0cJ2atbBhfy6LqAixmhjq2SlaZY9U.1
The seminar is open to all who are interested to learn more about STED, MINFLUX and the options that this advanced system offers – that also include confocal, Life-time imaging applications, Adaptive Optics and spectral imaging.
The Imaging & Optics Facility ( IOF ) at the Institute of Science and Technology Austria (ISTA) is hosting a seminar by Erin Tranfield (), about Correlative Light Electron Microscopy – CLEM.
Erin has more than 20 years of biological electron microscopy experience with expertise in room temperature EM, cryo-immobilization, electron tomography, CLEM, and is currently the of the VIB Bioimaging Core Ghent
Kind regards,
Your Imaging and Optics Facility
Image Analysis open-doors and Workstations maintenance
Struggling with image analysis, image acquisition or anything related to imaging or IOF instrumentation?
Feel free to stop by in rooms I01.O3.052a/b and ask your questions, doubts or advices at our image analysis open-doors! Now every 3rd Wednesday of the month.
IOF Team
PreSemester MICROSCOPY Course 2025/2026
The (pre-semester) microscopy course is organized by the Imaging & Optics Facility (IOF) and aims to generate the understanding of how to design your experiments using matching imaging techniques. As a red line we raise awareness of the importance of proper data acquisition and the timely image analysis assessments during essay establishment: timely assay-evaluation is critical to determine if the fundamental ‘properties of process of interest’ can be resolved and quantified by the used imaging approach. To this aim, participants will be introduced to contemporary microscopy techniques, with a focus on the methods that are available at ISTA campus. Lectures will cover microscopy-related principles of optics, principles of fluorescence, anatomy of the microscope, sample preparation, methods of optical sectioning, image acquisition methods, super-resolution technologies, basics of electron microscopy and the basics of data analysis.
We use the online Moodle Platform for the course handling, that can be reached via the following direct link:
→ 2025 Microscopy Course details on Moodle ←
PARTICIPATION
The online version is open to all. Use following credential to access the Zoom meeting:
(link will be provided)
REGISTRATION
Details for registration for the course will be made available on the GraduateSchool ISTA Athena.
Athena:
We use the online Moodle Platform for the course handling, that can be reached via the following direct link:
→ 2025 Microscopy Course details on Moodle ←
PARTICIPATION
- ‘Students’ join for 2 ECTS credits and will have mandatory participation in all lectures, on-system demonstrations (100% attendance required) and require to complete an exam at the end of the course (max 12 students)
- ‘Audit Students’ can join lectures and we will fill up vacant hands-on spots by order of registration (Zoom links will be shared to registered participants)
- All lectures can be followed in hybrid: credit-students attendance required!
Your Imaging & Optics Facility team
This workshop by Dr. Michael Loser from Ibidi will give an overview over the different available sample carriers, materials, surface treatments and culture conditions used for light microscopy.
When: 24.09.2025, 10:00-13:00
Where: Institute of Science and Technology Austria – ISTA, Am Campus 1, 3400 Klosterneuburg
Central Building (i01), Lecture Hall Mondi 1
Contact: iof@ist.ac.at
Registration:
Teledyne Photometrics will be hosting a camera technology workshop alongside the ISTA Imaging & Optics Facility. The workshop consists of an educational seminar and practical sessions where users can learn how to quantifiably, compare scientific camera images and optimise their use for specific applications.
THE TOPICS COVERED IN THE SEMINAR INCLUDE:
- Camera technology timeline
- Sensor architecture and design
- Understanding a camera datasheet
- Camera sensitivity
- Resolution
- Camera quality control
When: 25.09.2025,
13:00 – 14:00 Seminar
14:00 – 17:00 Practical Sessions
Where: Institute of Science and Technology Austria – ISTA, Am Campus 1, 3400 Klosterneuburg
Central Building (i01), Lecture Hall Mondi 3
Contact: iof@ist.ac.at
Registration:
Dear all,
Welcome to ISTA and thank you for your interest to start using laser equipment. This general laser safety training is meant for NEW scientists and staff that are planning to use laser based equipment and/or need access to a laser lab. New users of microscopes of the IOF machine park have to sign up for the IOF Newcomer training, where the laser safety to use microscopes is already included. This General laser safety training is planned for every first Monday of the month, at 15:00h and normally takes about 30 minutes
Request for “General Laser Safety Training”
This training is required by austrian law and will be held in person. When you sign up via email you will receive a room number for the next training.
Best regards,
The Laser Safety Office:
i: https://iof.pages.ist.ac.at/laser-safety
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