Nikon Ti2E stand equipped with iLas2 (GATACA) 360° / Ring / Azimuthal TIRF module. This module is using a pair of fast galvanometers to be able to spin the laser in the back focal plane of the objective several times during a single exposure time. That way artifacts such as fringes or rings caused by laser interference patterns are averaged out.
Description
Location: I24.U1.031 (moonstone building)
System description and specifications
- 360° TIRF imaging
- FRAP (with all laser lines)
- Photoactivation / -conversion (with all laser lines)
- Micro-patterning
- Widefield imaging
- laser angle sweep
Microscope and sample conditions
- Temperature controlled chamber
- CO2 supply
Light sources
- CoolLED pE300 White MB for widefield imaging and visual inspection of the sample
- Halogen lamp for visual inspection of the sample
- Omicron LightHUB Ultra laser system TIRF imaging and FRAP: 405nm, 488nm, 561nm, 640nm
Cameras
- 2 x Andor iXon Life 888 Back Illuminated EMCCD camera 1024 x 1024 active pixels, pixel size 13 μm x 13 μm, sensor area: 13.3 mm x 13.3 mm; 18.8 mm diagonal
- Front illuminated CMOS monochromatic camera for back focal plane imaging, 2048 x 1536 pixels, pixel size 3.45 µm
Objectives
- 20X / NA 0.75 / WD 1.00 mm Lambda CFI P –Apo (MRD00205)
- 60x/ NA 1.49/ WD 0.13 TIRF oil CFI Apochromat, with correction collar for coverglass thickness (0.13-0.20 mm), chromatic correction 435-1064nm (MRD01691)
- 100x/ NA1.49/ WD 0.12 oil TIRF oil CFI Apochromat, with correction collar for coverglass thickness (0.13–0.20 mm), chromatic correction 435–1064nm (MRD01991)
Dual-Cam filter option
Cairn TwinCam camera splitter
- TwinCam Cube dichroic mirror red/farred 643 nm
- TwinCam Cube dichroic mirror green/red,farred: 560 nm
- TwinCam Cube dichroic mirror blue/green: 488 nm
Bandpassfilters for TwinCam
- 450/50 ET Bandpass
- 525/50 ET Bandpass
- 595/50 H Bandpass
- 635 Longpass-Filter (Transmission 660 – 1200 nm)
700/75 H Bandpass
Filtercubes for Epifluorescence
- LED-FITC-A Filter Cube EX 474/27, EM 525/45
- LED-TRITC-A Filter Cube EX 554/23, EM 609/54
- LED-Cy5-A Filter Cube EX 628/40, BA 692/40
Software
NIS elements Ar 5.42.04
Computer: HP Z4 workstation
- Intel Xeon 3.9GHz 8Cores
- RAM:128GB DDR4
- 512GB OS Drive
- 2x 2TB Fast Storage
- 8TB Data Storage
- NVIDIA Quadro RTX5000 (16GB)
System manual and additional material is available on SeaFile (login required).
Additional information
| Building | i24 |
|---|---|
| Camera / Detector | EMCCD |
| Fluorescent Lightsource | Laser: 405±5 nm, Laser: 488±5 nm, Laser: 561±5 nm, Laser: 640±5 nm, LED: 355-365nm, LED: 430-450nm, LED: 545-555nm, LED: 640-650nm |
| System_Specification | Illumination: Fluorescence, Illumination: DIC, Incubation: heating chamber, Incubation: CO2/N2-gas mixer (hypoxia), Automated Stage, Z-piezo / galvo Stage, Focus Stabilization, Dual Camera (see detectors) |
| Technology / Application | 360° / Ring / Azimuthal TIRF, FRAP, In-vivo imaging, Micro-patterning, Photoactivation / -conversion, SAIM |
