DEMO: Leica DIVE CS: August 2020

PostedThe Bioimaging Facility isTThe bioimaging facility will host the  Leica SP8 DIVE CS multiphoton for a demonstration at IST Austriafrom mid-July until mid-August.  We offer half-day assisted slots during core facility hours from August 10 – August 28, 2020.

• Seminar: Daniel Smeets, Leica TCS DIVE multi-photon –  Tuesday, August 4th, 9:00 a.m., Heinzel Seminar Room / Office Bldg West.

Test-slots: Please send a request to bioimaging@ist.ac.at, with a short sample description and preference of day and time (morning / afternoon) →  confirmed slots are also listed in PPMS/schedule/MP/DEMO Leica DIVE

System specification: what can the system do or be used for: system brochure

• Multi-photon imaging: life, fixed, cleared + confocal option
• Fluorescent Lifetime Imaging (FALCON FLIM)
• Time gated quisition

SPECIFICATION:

• Fixed stage microscope Leica DM8 CS
• Multi-photon: IR Laser Spectra Physics InSight DeepSee (680-1300 nm) + Dual 1045
  • Spectral NDD-module 4Tune 4 channel (2 PMT, 2 HyD) 
• confocal: VIS-Laser 405nm,488nm, 552nm, 638nm diodes
  
  • 1 PMT, 2 GaAsP-HyD
• + Resonant scanner
• Cascaded IR black optical table with exchangable in vivo and in vivtro mounts, and additional:
  • Total 1300 mm x 1500mm;Scientifica MMBP “Motorised Moveable Base Plate” (MMBP): (0.1 micron steps / 50 mm range)
  • SuperZ Galvo stage (20nm steps / 1500 micron range)
• Single objective slider and additional 6x objective turret

OBJECTIVES

• • Objective HC PL APO 10x/0.40 CS2 Plan apochromatic  – Air / WD  2.2mm)
  • HC PL APO 63x/1.40 OIL CS Plan apochromat – oil / WD 0.14 mm
  • HC PL IRAPO 40x/1.1 W CORR.  water /  WD = 0.65 mm  → MP: 470-1200 nm
• 
  • Objective HC IRAPO L 25x/1.00 W mot IRAPO – water / WD  2.6 mm. ) – Motorized correction collar → MP: 470-1200 nm
•  
  • HC FLUOTAR L 16x/0.8 IMM motCORR VISIR. – water / WD  8.15 mm. ) – Motorized correction collar → MP: 470-1200 nm

TECHNOLOGY:

The system comes with a cascaded optical table that allows mounting of large samples.

DETECTION:  the DIVE system come with the uniquer non-descanned 4-tune technology system that allows to dynamically select the emission spectrum when imaging in Multi-photon. This is especially interesting for new dyes and to select different spectral regions for simultaneous acquisition and unmixing of fluorophores, such as rainbow labeling strategies.