Abberior Mirava polyscope (STED / Minflux)

Posted onAuthorGabriel Krens

Abberior Mirava polyscope (STED / Minflux)

The Abberior Miriava Polyscope comes with a multitude of imaging modalities that allow imaging beyond the diffraction limit of light and approach molecular resolution: Confocal (200 nm) > Matrix detector (70 nm) > 2D / 3D STED (20 nm) > Minflux (3.2 nm). Additional advanced technologies to correct for aberrations. The system is equipped with a incubation chamber, stage-heater an options for humidity – CO2 and O2 control.

Imaging Modes:

  • Confocal: up to 200nm resolution
  • Matrix: Array detector that allows up to 70nm resolution
  • STED : 2 color STED in 2D (20nm) or 3D (< 100nm), with
  • Minflux: nanometer-scale resolution (3.2 nm)

Imaging Applications:

  • Adaptive Optics (Rayshape): Deformable mirror-based correction of spherical aberrations for all beams
  • Adaptive illumination for STED (Flexposure): Light dose minimization to leverage live cell imaging and minimize photo-bleaching
  • Life Time Imaging (Timebow): Life time measurement and Life Time filtered STED
  • Spectral detection (400-800nm; Matrix)
  • Deconvolution using the Truesharp module
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Description

Location: I24.U1.031 (Moonstone Building)

Specification

  • Evident/Olympus  IX83 stand
  • Incubation chamber for humidity – CO2 and O2 control.
  • 7 excitation laser lines (405, 488, 561, 642nm)
  • Automated XY-stage including universal sample holder (well plates, 35mm dishes, and slides)
  • Additional 200 micron piezo driven z-axis for fast imaging
  • Autofocus Z-drift compensation (confocal / STED)
  • Multi-position & tiling

Objectives (Evident/Olympus)

  1. 10x / NA 0.4 / 3.1mm (UPLXAPO10X), Dry → sample navigation
  2. 20x /NA 0.95 / 0.6mm (UPLXAPO20X), Dry → confocal
  3. 40x / NA 0.95 / 0.18mm (UPLXAPO40X), Dry → confocal
  4. 60x / WD: 0.15, NA: 1.42 (UPLXAPO60xO), Oil → confocal & STED
  5. 60x / NA 1.3, (UPLSAPO60X S), Silicone Oil → confocal & STED
  6. 60x / NA 1.2,  (UPLSAPO60XW) Water → confocal & STED

Light Sources

  • 7x excitation laser lines (405, 440*, 485*, 518*, 561*, 640*, 685* nm) – * pulsed lasers#
  • 1x CW 640 nm for Minflux excitation
  • 2x ultra high power pulsed STED lasers (775 & 589nm)
  • 16x CoolLED lightsource

Detectors

  • 4x APD detectors (Avalanche Photo-Diode)
  • 1x Matrix detector
  • 1x Transmitted light detector

Software

Abberior Lightbox & Imspector

  • SLM for abberation correction and 2D/3D STED modulation
  • FLEXPOSURE adaptive illumination package for low-light live cell super resolution
  • Push button auto alignment
  • RAYSHAPE Adaptive Optics System with deformable mirror correction for all excitation beams
  • TIMEBOW software package for FLIM measurements

Computers Workstations

Microscopy Workstation: xxx

  • Graphics Card
  • Hard Drive Extension 1
  • CPU I
  • Storage and Data Analysis

Additional information

Building

i24
Technology / Application

FLIM, Time-lapse recording, Multi-position, In-vivo imaging, Spectral Imaging, Super Resolution (SR), SR: MINFLUX (<5nm), SR: SMLM (20nm), SR: STED (±20-40nm), Time Gating
System_Specification

Illumination: Fluorescence, Incubation: heating chamber, Incubation: heating stage, Incubation: CO2/N2-gas mixer (hypoxia), Automated Stage, Z-piezo / galvo Stage, Focus Stabilization
Objective Specification

10x, 20x, 40x, 60x / 63x, Oil, Silicone, Water
Fluorescent Lightsource

Laser: 685±5 nm, Laser: 405±5 nm, Laser: 445±5nm, Laser: 488±5 nm, Laser: 514±5nm, Laser: 561±5 nm, Laser: 640±5 nm

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