PreSemester MICROSCOPY Course 2025/2026

Posted onAuthorGabriel Krens

PreSemester MICROSCOPY Course 2025/2026

September 22, 2025 – September 30, 2025

reSemester MICROSCOPY Course 2025/2026

The (pre-semester) microscopy course is organized by the Imaging & Optics Facility (IOF) and aims to generate the understanding of how to design your experiments using matching imaging techniques. As a red line we raise awareness of the importance of proper data acquisition and the timely image analysis assessments during essay establishment: timely assay-evaluation is critical to determine if the fundamental ‘properties of process of interest’ can be resolved and quantified by the used imaging approach. To this aim, participants will be introduced to contemporary microscopy techniques, with a focus on the methods that are available at ISTA campus. Lectures will cover microscopy-related principles of optics, principles of fluorescence, anatomy of the microscope, sample preparation, methods of optical sectioning, image acquisition methods, super-resolution technologies, basics of electron microscopy and the basics of data analysis.

We use the online Moodle Platform for the course handling, that can be reached via the following direct link:

→ 2025 Microscopy Course details on Moodle ←

PARTICIPATION

The online version is open to all. Use following credential to access the Zoom meeting:

Zoom Link

Meeting ID: 665 3699 3974
Passcode: 2025MIC

SCHEDULE:

The Imaging & Optics Facility is providing the ‘Annual Microscopy Course’ – split over 2 weeks:

– week 1:

  • Sep 22 (Monday) 13.30-17.00 – Mondi 3
    • Lecture: Anatomy of the microscope
    • Lecture: Intro to optics
  • Sep 23 (Tuesday) 9.00-17.15 – Mondi 3
    • Lecture: Intro to Fluorescence
    • Lecture: Sample Preparation
    • System Demo: Widefield TIRF – at the microscopes
    • System Demo: Cytometry – at the systems
  • Sep 24 (Wednesday)  9.00-17.15 – Mondi 3
    • Lecture: Optical Sectioning
    • Scientific Cameras
    • System Demo: Confocal + Airyscan
    • System Demo: Spinning Disk
  • Sep 25 (Thursday) 9.00-12.30 – Mondi 3
    • System Demo: Lightsheet
    • System Demo: Multi-photon
  • Sep 24 (Friday) 9.00-12.30 – Mondi 3
    • Lecture: Superresolution technologies & deconvolution
    • Lecture: Intro to Electron Microscopy- lecture

– week 2:

  • Sept 29 (Monday) 9.00-12.30 – Mondi 3
    • Lecture: What is a digital image?
    • Demonstration: Introduction to Image analysis (basics of ImageJ/FIJI)
    • Hands-on: ImageJ/FIJI: Image Analysis
  • Sept 30 (Tuesday) 9.00-12.30 – Mondi 3
    • Lecture: Machine Learning in Image Analysis
    • Demonstration: What to use for what – a software overview
    • Lecture: Introduction to Laser Safety
    • Recap: Recap & Exam details

 

REGISTRATION

Details for registration for the course will be made available on the GraduateSchool ISTA Athena.

Athena:

  • Credit 2 ECTS (full course): C_OTH-301_F25 (link)
  • Credit 1 ECTS (lectures only): C_OTH-302_F25 (link)
  • Audit: A_OTH-301_F25 (link)

We use the online Moodle Platform for the course handling, that can be reached via the following direct link:

→ 2025 Microscopy Course details on Moodle ←

PARTICIPATION

  • ‘Students’  join for 2 ECTS credits and will have mandatory participation in all lectures, on-system demonstrations (100% attendance required) and require to complete an exam at the end of the course (max 12 students)
  • ‘Audit Students’ can join lectures and we will fill up vacant hands-on spots by order of registration (Zoom links will be shared to registered participants)
  • All lectures can be followed in hybrid: credit-students attendance required!

 

Your Imaging & Optics Facility team

Am Campus 1
Klosterneuburg, 3400
Austria
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