Location: BFB, room I04.03.011 (Temporary location – move to i24 in late 2024)
The BD FACSMelody is the smaller sister of our Aria III. While it provides less lasers and channels, it can be used autonomously by our users and is meant especially for prolonged bulk sorting.
Book in PPMS or Request a training
Software: FACS Chorus
Available Channels:
- 405 nm violet laser
- DAPI, Pacific Blue, Horizon V450 (448/45)
- BD V510, V500, (500 LP, 528/45)
- 488 nm blue laser:
- FITC, EGFP, Alexa488 (507LP, 527/32)
- PerCP-Cy5-5, 7-AAD (665LP, 700/54)
- 561 nm yellow/green laser:
- PE, DsRed, tdTomato, Propidium Iodide (PI) (582LP, 582/15)
- PE-Texas Red, mCherry (605LP, 613/18)
- PE-Cy5, PE-Cy5-5 (665LP, 697/58)
- PE-Cy7 (752LP, 783/56)
Available Nozzle sizes:
FACSMelody only provides one set-up. If you need different settings, consider using our BD FACSAria III
| Nozzle size* (μm) |
Sheath pressure (psi) |
Frequency** (kHz) |
Cell types |
| 100 | 23 | 34 | Tissue cells in general, Neurons, Macrophages, tissue DC, Stem cells, cell lines |
*max cell size ~ 1/5 of nozzle size
**max. event rate is ~1/3 or frequency
System manual and additional material is available on SeaFile (login required).
Contact for application and experiment planning:
-
- ticket: cytometry@ist.ac.at