Location: BFB, room I04.EG.017
The BD FACS Aria III can only be used in the presence of an operator (user). Startup, shutdown and system configuration are exclusively performed by trained Imaging and Optics Facility staff in order to prevent instrument damage and down-times.
Book in PPMS or Request a training
Software: FACS Diva 9.7.
Available Channels:
- 488 nm blue laser:
- FITC, EGFP, CFSE (502LP, 530/30)
- PerCP-Cy5-5, 7-AAD (655LP, 695/40)
- 561 nm yellow/green laser:
- PE, tdTomato, Propidium Iodide (PI) (582/15)
- PE-Texas Red, mCherry (600LP, 610/20)
- PE-Cy5 (630LP, 670/14)
- PE-Alexa Fluor 700, PE-Cy5-5 (685LP, 710/50)
- PE-Cy7 (735LP, 780/60)
- 633 nm red laser:
- APC, AlexaFluor 647 (660/20)
- AlexaFluor 700 (690LP, 730/45)
- APC-Cy7, APC-H7 (755LP, 780/60)
- 405 nm violet laser (OR 375 nm UV laser):
- DAPI, Pacific Blue, Horizon V450 (450/40)
- BV510, V500, (502 LP, 510/50)
- Qdot 605 (600LP, 610/20)
Additional filters are available and can be installed upon request → please contact the facility at least 1 week in advance, as custom device configurations require the setup of a new calibration baseline.
Available Nozzle sizes:
| Nozzle size* (μm) |
Sheath pressure (psi) |
Frequency** (kHz) |
Cell types |
| 70 | 70 | 87 | Non-activated T cells, B cells, Platelets, Bacteria |
| 85 | 45 | 47 | Activated T cells, PLasma cells, NK-T cells, NK-cells, Monocytes, mDC, pDC, Stem cells |
| 100 | 20 | 30 | Tissue cells in general, Neurons, Macrophages, tissue DC, Stem cells, cell lines |
| 130 | 10 | 12 | Problematic cell-types using other nozzle sizes |
*max cell size ~ 1/5 of nozzle size
**max. event rate is ~1/3 or frequency
System manual and additional material is available on SeaFile (login required).
Contact for application and experiment planning:
-
- ticket: cytometry@ist.ac.at