Zeiss LSM 800-03 inverted

location: I06.U1.405

Axio Observer.Z1, Inverted Confocal microscope, ZEN 3.5 with experiment designer.

Overnight acquisitions: Please reboot system before and start system in DEBUG MODE (+ screenshot script): do not forget to SAVE the debug log-file in case of a crash!

Light sources:

  • Laser lines 405, 488, 561, 640 nm
  • CoolLED p300 SB light
  • ESID LED
  • 2x MA-PMT channels simultaneously
  • Multiple position imaging

Detectors: 2 channels simultaneously (more sequentially)

  • 2x MA-PMT: Multi Alkali Photomultiplier Tube detectors with free choice of the spectral range. (Uses a tunable short-pass and band-pass filter in front of the PMTs)
  • 1x ESID+LED light source: Electronically switchable illumination and detection module

Motorized stage for multiple position imaging

  • Motorized stage for multiple position imaging
  • Multi-slide holder, dish holder, single slide holder


Objectives (M27 thread)

  1. Plan-Apochromat 5x (Calibration objective-not mounted)
  2. Plan-Apochromat 10x/0.45
  3. Plan APOCHROMAT 20x/0.8 (420650-9901), WD=0.55 mm (D=0.17 mm), WD=2.1 mm
  4. Plan-Apochromat 40x/1.2 Water
  5. Plan-APOCHROMAT 63x/1.4 Oil

Software: Zen 3.5

  1. ZEN Module Experiment Designer Hardware
  2. ZEN Module Tiles & Positions

Computer:

  • Intel® Core™ i5-6500 CPU @ 3.2GHz
  • 64GB RAM
  • 3.38TB data drive

Contact persons:

Manual

See detailed description and manual: